Zoo/PhytoImage Examples: ------------------------ There is a series of example datasets available to demontrate Zoo/PhytoImage features. These can be downloaded separately from http://www.sciviews.org/zooimage In any case, you should start practicing with these datasets, following respective short tutorials provided. They demonstrate how to analyze images of different origins: - MicroCol24-example: micro/macrophotographies with any kind of digital camera with no calibration, or only spatial calibration and 24bit color. - ScanCol24-example: stained plankton scanned at low resolution (600/800dpi) with any kind of flat-bed scanner; 24bit color. - ScanG16-example: high resolution (1200/2400dpi) 16bit gray images scanned in transparency mode with an Epson 4870/4990 scanner, using Vuescan. Full calibration of the background (done automatically by the scanner), gray levels using neutral gray photographic filters, and spatial calibration (fixed resolution). - MacroG16-example: high resolution (1000-3000dpi) 12bit gray raw images obtained with a reflex digital camera and a professional macro lens. These photographies were acquired with a Canon 20D (8 megapixels) camera, a Canon 100mm f/2.8 Macro lens, a Canon 580Ex flash, and two white reflectors at 45° each to light the samples from behind (transparency mode). Full calibration of the images through a blank-field image (calibration of the background), a picture of neutral gray photographic filters (calibration of gray levels) and a picture of a 2mm scale (spatial calibration). - FlowCAM-example-FIT-IMS: data acquired with an old FlowCAM analog model. - FlowCAM-example-FIT-VIS: data acquired with a recent digital FlowCAM and the Visual Spreadsheet software. - Scan24-train&data: an example of a simple training set; color scanned images. - ScanG16-train&data: an example of a more detailed training set, and a second version partly reworked. - MacroG16-train&data: another training set, done with macrophotographies. - PlanktonJ-example: a .zid (Zoo/PhytoImage Data) file to be used with PlanktonJ. Explore Zoo/PhytoImage: ----------------------- Start with the 'ScanCol24-example' datasets, which is the simplest analysis one can do (one picture per sample, one .zim file -metadata file- per image). Image processing should be straightforward and finalize it by building the two corresponding .zid files. It is also a good illustration of images you can get with almost any flatbed scanner available out there, using a Petri dish to hold your sample and haematoxylin staining to better separate the silhouettes. You should try also 'ScanCol24-train&data' which contains a simple training set. Learn how to import it, train a classifier and then, use the classifier to calculate variables of interest for your samples, display and export final results. Next, 'MicroCol24-example' is a small dataset that pretty well illustrates the use of the Zoo/PhytoImage compiler to create a large number of .zim metadata files. It uses both a template in 'ImportTemplate.zie', and a table of characteristics for each sample stored in an ASCII *.txt file to automatize importation of images and data into a suitable format. The .xls Excel version of the table is also provided. It is easier to edit, but you must export it into a text file (the .txt version) before you can use it in the current version of Zoo/PhytoImage. The photographies are taken with a microscope and a digital camera (a widely available digitizing system). Once you can run these three examples and understand the logic behind, you should be ready to use Zoo/PhytoImage with your own data. However, it is worth studying the other, more complex, examples dealing with high-resolution and fully calibrated pictures: 'MacroG16-example' and 'ScanG16-example'. Corresponding example training sets and series of samples are in 'MacroG16-train&data' and 'ScanG16-train&data'. Switch to them, after you understand how to create .zid files from your images.