FlowCAM-example-FIT-IMS: ------------------------ This is an example of a very small dataset obtained with the analog FlowCAM (see FlowCAM-example-FIT-VIS for an example with the more recent digital version of the FlowCAM). It is too limited for extensive analysis, but it is fine just to test how FlowCAM data can be imported and analyzed in Zoo/PhytoImage To run this example: -------------------- - Download and unzip "FlowCAM-example-FIT-IMS.zip" in your 'ZooPhytoImage Examples' directory, or anywhere you like to place it. - Start ImageJ directly and go to plugins -> FIT -> FIT IMS. Select the .fcm file, then use default values and look at the analysis as it proceeds. - Once the analysis is done, you can close ImageJ and switch to the Zoo/PhytoImage assistant. Click on the fourth button to finalize the .zid file corresponding to this analysis. You should end up with one .zid file (Zoo/PhytoImage Data) plus all the original files zipped and moved to the _raw subdirectory. What next...: ------------- The .zid files are key files in Zoo/PhytoImage. They contain everything you need for manual and automatic identification of the particles and for the calculation of summary statistics (abundances, biomasses, size spectra, etc.). At this point, you can archive (on DVD, external hard disks, etc.) the original data that are now in the _raw subdirectory to free space on your machine, and safely work with the .zid files only. This short tutorial demonstrates, thus, how to obtain such .zid files from FlowCAM data. With so few samples, you cannot do much more, but you are supposed to collect more data, then to train a classifier, check its performances and finally use it to identify particles in your samples. For examples with more data, switch to the files whose name ends with 'train&data'.